On May 4 of 2016, within the promotion of practices in detection and control of invasive exotic plant species in roads, six elements of the University of Porto team traveled to a section of National route nº4 (EN4), to locate four species of invasive exotic plants (Acacia dealbata, A. melanoxylon, Robinia pseudoacacia e Ailanthus altissima). In the period of greatest solar incidence, between 11 am and 03 pm to avoid the influence of the shadow were identified 17 points. The location was collected using a GPS Real-Time Kinematic (RTK). The reflectance data collected using a portable spectroradiometer (Handheld 2, ASD Instruments, Boulder, CO, USA) that records spectral data from 325 to 1075 nm which allows to obtain pure spectral signatures of the invasive species. The photographs were taken with a Raptor Photonics OWL 320 camera, which records images on infrared band of the spectrum (900-1700 nm). At last, were collected biological material of the species to further confirmation in the laboratory. The data collected in this field trip are going to be used in the characterization of the spatial distribution of the invasive plant species along the roads using remote sensing methods.